Figure 3.

Images and quantitation of ex vivo autoradiography and S1R staining of injured and uninjured rat sciatic nerve sections. (A) Representative autoradiography images of injured (upper panel) versus uninjured (lower panel) nerve sections, H&E staining of the same section used for autoradiography, and immunohistochemical staining of S1R in injured versus uninjured nerves. Whole nerve and muscle images were taken at 1× magnification, scale bar = 2.0 mm; neuroma images were taken at 40× magnification, scale bar = 50 µm. (B) Quantitative bar graphs showing the % area occupied by S1R staining and the mean pixel/S1R-staining intensity in injured (n = 4) versus uninjured (n = 4) nerves. Error bars represent standard error of the mean value, *p<0.05. (C) Double immunofluorescence staining of injured (two upper panels) and uninjured (two lower panels) sciatic nerves. Schwann cell (using anti-S100β antibody) is shown in green, whereas S1R immunostaining (using anti-S1R antibody) is shown in red. DAPI (4',6-diamidino-2-phenylindole) nuclear counterstaining is shown in blue, and the merged image of all three stains for both the injured and uninjured nerves is shown on the far right of each row. All images were taken at 40× magnification, scale bar = 10 µm.