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. 2017 Jul 8;7(11):2837–2848. doi: 10.7150/thno.18968

Figure 8.

Figure 8

NIRF imaging of the Cy5.5-H2BP probe in the photothrombotic stroke mice. (A) In vivo Cy5.5-H2BP imaging results in mice 1-24 h after cerebral ischemia. (B) The statistical results for the TBR signal at each time point was calculated for the Cy5.5-H2BP and Cy5.5-H2CP (control) probes from the NIRF images of the ischemic/contralateral hemispheres following in vivo imaging in mice. (C) T2-MR in vivo and in vitro imaging results and TBR numerical statistics for the brain tissue of mice injected with the Cy5.5-H2BP and Cy5.5-H2CP (control) probes 24 h after cerebral ischemia.(D) Intensity of the NIRF signals in the brain, heart, liver, spleen, lung and kidney. The data are represented as the mean±SEM, n=6, *P<0.05, **P<0.01, ***P<0.001. (E) Detection of the Cy5.5-H2BP probe and Cy5.5-H2CP probe in the ischemic brain sections. (Upper) MRI-T2 imaging of the probes injected into the mice 24 h after photothrombotic stroke. (Lower) Confocal scanning analysis of brain tissues sections from mice injected with the Cy5.5-H2BP probe and Cy5.5-H2CP probe (scale bar, 50 μm). C, P and I represent the ischemic core, peri-ischemic zone and intact zone, respectively.