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. 2017 Jul 8;7(11):2863–2877. doi: 10.7150/thno.19353

Figure 3.

Figure 3

cZNF609 regulates endothelial cell function in vitro (A) HUVECs were transfected with scrambled siRNA (Scr), siRNA-1, siRNA-2, siRNA-3, or left untreated (Ctrl) for 48 h. qRT-PCRs were conducted to detect cZNF609 and ZNF609 mRNA expression (n=4, *P<0.05). (B) Cell viability was detected using MTT method (n=4, *P<0.05). (C) Cell proliferation was detected using EdU detection kit (Ribobio, Guangzhou, China) to analyze the incorporation of EdU during DNA synthesis (n=4, *P<0.05). Scale bar, 200 μm. (D) Transwell assay and quantification analysis was conducted to detect HUVEC migration (n=4, *P<0.05). Scale bar, 20 μm. (E) HUVECs were seeded on the matrigel matrix. The tube-like structures were observed 24 h after cell seeding. Average length of tube formation for each field was statistically analyzed (n= 4, *P<0.05). Scale bar, 100 μm. All data were from at least three independent experiments.