Table 1.
Comparison of different radiofluorination methods for labeling the heat-sensitive protein HSA.
| Method | (±)-[18F]AlF(RESCA)-HSA | [18F]F-Py-HSA [36] | Cu(I)mediated 1,3-dipolar [3+2]cycloaddition [37] |
|---|---|---|---|
| RCY* | 52-63% (n=3) | 18-35% (n=30) | 15-20% |
| RCP | > 98% | > 98% | > 95% |
| Synthesis time** | < 30 min | 90 min | 120 min |
| Tb | 8.6 h (in rats) | 4.8 h# (in rats) | 31 min (in mice) |
RCY: radiochemical yield; RCP: radiochemical purity; Tb: blood biological half-life
*Calculated based on the amount of radioactivity eluted from disposable desalting column relative to the starting activity of [18F]F-**Starting from [18F]F- # Data for [18F]F-Py-RSA (rat serum albumin)