Fig. 5.

Ndc10p is required for spindle stability during anaphase and cell separation. Wild-type and ndc10–1 cells were arrested in G₁ with α-factor and released at 37°C. Spindles were categorized as being monopolar, short, medium, long, or broken/disassembled based on morphology and length of GFP-Tub1p fluorescence (see Materials and Methods). (A) Spindle lengths of wild-type cells released from G₁ arrest at 37°C. (B) Spindle lengths of ndc10–1 cells released from G₁ arrest at 37°C. (C) Time-lapse fluorescence images of GFP-Tub1p in ndc10–1 cells grown at 37°C. (Bar, 5 μm.) (D) Quantitation of spindle length for cell presented in C.