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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: J Cell Physiol. 2017 May 31;233(4):2781–2790. doi: 10.1002/jcp.25979

Figure 4. Commonly applied read-outs for somatic decline upon DNA damage induction (see Methods).

Figure 4

(A) Life span assay of wild type and xpa-1 mutants irradiated at day 1 of adulthood. (B) Pumping assay performed in adult-irradiated wild type and xpa-1 mutants 72 hours post-exposure. Error bars show SEM. (C) DAF-16::GFP nuclearization upon UVB-irradiation in xpa-1 mutant animals synchronized at L3 stage. The images are representative for the categories cytosolic, partially nuclear and fully nuclear. Size bars correspond to 100 µm. (D) Germline DNA damage induced stress resistance (GDISR) upon heat stress. Worms were treated either with 0 Gy (Control) or 90 Gy of IR at L4 larval stage before being shifted to 35°C 48 hours later. Experiment was done in biological triplicates. Error bars indicate standard error of the mean.