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A, Purified SecYAEG complex analyzed by SDS-PAGE and Coomassie Brilliant Blue staining; B, Protein translocation assay using SecYAEG-reconstituted or SecYEG-reconstituted liposomes in the presence or absence of SecA ATPase; protease-resistant translocated substrate, proOmpA-His6-Myc, was detected by anti-Myc immunoblotting. C, Schematic depiction of the protein translocation systems in vitro.
