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. Author manuscript; available in PMC: 2017 Aug 21.
Published in final edited form as: Oncogene. 2016 Oct 31;36(18):2553–2564. doi: 10.1038/onc.2016.408

Figure 3. Deficiency in Smarcal1 does not alter thymocyte sensitivity to radiation.

Figure 3

A) Representative dot plots of littermate matched thymic T cells without or at the indicated times following a single dose of 1.75 Gy of IR (left). Mean of data from seven independent experiments (right). B) Total thymic cellularity in unirradiated littermates. C) CD4/CD8 DP thymocytes not subjected to IR or at the indicated times after IR. D-F) Thymi harvested 24 hours after littermates were subjected to a single dose of 1.75 Gy of IR. D) Cleaved caspase 3/7 activity and Annexin V positivity in thymocytes measured by flow cytometry. E) Immunofluorescence for γH2AX was quantified; thymocytes with >5 γH2AX foci graphed. F) Mean BrdU incorporation of three independent litters. Error bars represent SEM; n denotes the number of mice.