Table 2.
Differentially expressed genes as a result of CRISPR-mediated gene 33 knockout and 8 weeks of Cr(VI) treatment: major functional groups
| Function | Gene symbol | Gene name | FC |
|---|---|---|---|
|
| |||
| EMT and Metastasis | ZEB2 | zinc finger E-box binding homeobox 2 | −2.4 |
| NUPR1 | nuclear protein transcription regulator 1 | −4.0 | |
| ErbB3 | erb-b2 receptor tyrosine kinase 3 | −2.6 | |
| GPNMB | glycoprotein nmb | −2.6 | |
| Cell adhesion | CDH4 | cadherin 4 | +2.6 |
| ITGA4 | integrin subunit alpha 4 | +2.6 | |
| CAV2 | caveolin 2 | +1.9 | |
| APBB1IP | amyloid beta precursor protein binding family B member 1 interacting protein | +4.9 | |
| LAMA4 | laminin subunit alpha 4 | +3.2 | |
| PCDHGA11 | protocadherin gamma subfamily A, 11 | +4.0 | |
| COL5A2 | collagen type V alpha 2 chain | +2.6 | |
| PKP1 | plakophilin 1 | +2.6 | |
| PDPN | podoplanin | +7.5 | |
| ICAM1 | intercellular adhesion molecule 1 | −2.3 | |
| ROBO1 | roundabout guidance receptor 1 | −2.9 | |
| Oxidative stress | GSTM1 | glutathione S-transferase mu 1 | +6.3 |
| PPARD | peroxisome proliferator activated receptor delta | −2.1 | |
| CYGB | cytoglobin | +3.7 | |
| GPX3 | glutathione peroxidase 3 | +3.7 | |
| CHAC1 | ChaC glutathione specific gamma-glutamylcyclotransferase 1 | +2.3 | |
| CLU | Clusterin | +2.1 | |
| GPCR signaling | ADGRE1 | adhesion G protein-coupled receptor E1 | −9.1 |
| BDKRB1 | bradykinin receptor, beta 1 | −3.0 | |
| RGS4 | regulator of G-protein signaling 4 | +2.6 | |
| MRGPRF | MAS related GPR family member F | −2.6 | |
| GPR162 | G protein-coupled receptor 162 | −3.2 | |
| RTK signaling | ErbB3 | erb-b2 receptor tyrosine kinase 3 | −2.6 |
| FGF1 | fibroblast growth factor 1 | −2.0 | |
| GFRA1 | GDNF family receptor alpha 1 | +2.1 | |
| IGFBP4 | insulin like growth factor binding protein 4 | +2.5 | |
| Protein phosphatase | PPP1R3C | protein phosphatase 1 regulatory subunit 3C | −2.3 |
| WNT signaling | WNT7B | Wnt family member 7B | +2.8 |
| DIXDC1 | DIX domain containing 1 | +2.6 | |
| TCF4 | transcription factor 4 | +3.2 | |
| TLR signaling | ZG16B | zymogen granule protein 16B | +2.8 |
| TNF signaling | TNFRSF11A | tumor necrosis factor receptor superfamily member 11a | +2.5 |
| TNFRSF21 | tumor necrosis factor receptor superfamily member 21 | +2.5 | |
| TNFRSF9 | tumor necrosis factor receptor superfamily, member 9 | −3.0 | |
| Vesicle trafficking | CAV2 | caveolin 2 | +1.9 |
| SYTL3 | synaptotagmin like 3 | +2.6 | |
| STON2 | stonin 2 | −2.6 | |
| AMPH | amphiphysin | −25.0 | |
| histone | HIST1H4F | histone cluster 1, H4f | +3.5 |
| HIST1H4I | histone cluster 1, H4i | −2.3 | |
| Calcium signaling | PDE1C | phosphodiesterase 1C | +4.0 |
| S100A2 | S100 calcium binding protein A2 | +2.6 | |
| Long non-coding RNA | MEG3 | maternally expressed 3 | +4.3 |
| LINC01116 | long intergenic non-protein coding RNA 1116 | +2.0 | |
| LINC00958 | long intergenic non-protein coding RNA 958 | +5.7 | |
| Cytoskeleton | KRT34 | keratin 34 | −3.0 |
| SHROOM4 | shroom family member 4 | +3.0 | |
| Transcriptional regulator | KRABOX1 | KRAB box domain containing 1 | −100.0 |
| ZNF518B | zinc finger protein 518B | +3.0 | |
| NUPR1 | nuclear protein transcription regulator 1 | −4.0 | |
| TCF4 | transcription factor 4 | +3.2 | |
| Ubiquitination | HERC3 | HECT and RLD domain containing E3 ubiquitin protein ligase 3 | +2.3 |
| UCHL1 | ubiquitin C-terminal hydrolase L1 | +147.0 | |
| FBXO32 | F-box protein 32 | +3.0 | |
| Carrier protein | MLPH | Melanophilin | +2.5 |
| SLC38A5 | solute carrier family 38 member 5 | −7.7 | |