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. Author manuscript; available in PMC: 2018 Mar 16.
Published in final edited form as: Chembiochem. 2017 Feb 2;18(6):506–510. doi: 10.1002/cbic.201600669

Figure 8.

Figure 8

UPR response. Western blot data from HCT116 cells treated with 0.5–10 μM of 3 for 12 h, collected, lysed and blotted using the ascribed mAb as given by XBP1-s (12782S, Cell Signaling), GRP78 (3183S, Cell Signaling), pEIF2α (9721S, Cell Signaling), EIF2α (9722S, Cell Signaling), ATF-4 (11815S, Cell Signaling), 1:1000 GAPDH (sc-32233, Santa Cruz Biotechnology), and 1:3000 GADD 34 (sc-825, Santa Cruz Biotechnology). GAPDH was used as a loading control, 5 μM MG132[20] as a positive control (+), and DMSO alone as a negative control (–).