Table 1.
Identification of antagonistic bacterial isolates on the basis of 16S rRNA and their antimicrobial activity against human pathogenic bacteria.
|
bAntibacterial activity against | ||||||||
|---|---|---|---|---|---|---|---|---|
| Strain lab no | Accession numbers | Closely related type strain | a% identity | E. coli | MRSA | P. faecium | P. faecalis | P. aeruginosa |
| Root | ||||||||
| EA 1 | KR812389 | Pseudomonas parafulva AJ 2129T | 98.60 | − | − | − | + | − |
| EA 2 | KR812390 | Leucobacter chromiiresistens JG 31T | 98.60 | − | − | + | + | + |
| EA 3 | KR812391 | Prolinoborus fasciculus CIP 103579T | 98.30 | − | − | − | − | + |
| EA 4 | KR812392 | Pantoea dispersa LMG 2603T | 97.90 | − | − | + | + | − |
| EA 5 | KR812393 | Erwinia toletana CECT 5263T | 98 | + | + | − | + | ++ |
| EA 6 | KR812394 | Erwinia piriflorinigrans CFBP 5888T | 98 | − | + | + | − | − |
| EA 7 | KR812395 | Carnobacterium viridans MPL-11T | 97.10 | − | − | + | − | − |
| EA 8 | KR812396 | Pseudomonas cremoricolorata NRIC 0181T | 99.30 | ++ | − | − | − | − |
| EA 9 | KR812397 | Bacillus subtilis subsp. inaquosorum KCTC 13429T | 98.90 | − | − | − | + | + |
| EA 10 | KR812398 | Bacillus endophyticus 2DTT | 98.70 | ++ | − | − | − | − |
| EA 11 | KR812399 | Bacillus methylotrophicus CBMB205T | 97.60 | − | + | − | − | − |
| EA 12 | KR812400 | Bacillus aryabhattai B8W22T | 99.70 | − | − | − | + | + |
| EA 13 | KR812401 | Staphylococcus sciuri subsp. sciuri DSM 20345T | 99.50 | − | + | − | − | − |
| EA 14 | KR812402 | Terribacillus aidingensis YI7–61T | 98.90 | − | − | + | − | − |
| EA 15 | KR812403 | Staphylococcus lentus ATCC 29070T | 99.40 | − | − | + | − | − |
| EA 16 | KR812404 | Leucobacter chromiiresistens JG 31T | 98.40 | + | ++ | − | − | ++ |
| EA 17 | KR812405 | Carnobacterium inhibens K1T | 98.60 | − | + | − | − | − |
| Leaf | ||||||||
| EA18 | KR812406 | Bacillus endophyticus 2DTT | 98.60 | ++ | + | + | + | + |
| EA 19 | KR812407 | Bacillus atrophaeus JCM 9070T | 99.80 | − | + | − | − | − |
| EA 20 | KR812408 | Pseudomonas chlororaphis subsp. piscium JF3835T | 97.80 | + | − | − | − | − |
| EA 21 | KR812409 | Bacillus aryabhattai B8W22T | 99.70 | − | − | + | − | − |
| EA 22 | KR812410 | Brevibacterium halotolerans DSM 8802T | 99.50 | − | + | + | − | − |
| Fruit | ||||||||
| EA 23 | KR812411 | Pseudomonas entomophila L48T | 99.60 | − | + | − | − | − |
| EA 24 | KR812412 | Leucobacter chromiiresistens JG 31T | 99.50 | − | ++ | − | − | − |
a
Based on the results of partial 16S rRNA gene sequence analysis of all strains.
b
The antibacterial activity was determined by in vitro Double agar overlay method. The activity was estimated after 24 h incubation at 28 °C by measuring the clear zone of bacterial inhibition: +, <3 mm; ++, between 4 and 5 mm; −, no activity.