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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: J Cell Physiol. 2017 May 11;232(12):3798–3807. doi: 10.1002/jcp.25862

FIGURE 1.

FIGURE 1

DHT counteracts osteoclastogenesis driven by forced RUNX2 expression. Newborn Mouse Calvarial Osteoblasts (NeMCO) were transduced with lentiviruses encoding dox-inducible RUNX2. NeMCO/Rx2dox were co-cultured with primary murine splenocytes in the presence of dox and/or DHT as indicated. (a and b) Osteoclasts were identified based on TRAP staining (a; scale bar = 100 μm) and enumerated (b). Results are from one of three experiments with similar results (Mean ± SD; n = 3; *p < 0.05). (c) Western blot analyses with anti-RUNX2 and anti-ACTIN antibodies. (d–f) Levels of the indicated mRNAs were determined by RT-qPCR (Mean ± SD; n = 3; *p < 0.05). C, control; D, dox; A, DHT; DA, dox plus DHT