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. 2017 Aug 18;7:8703. doi: 10.1038/s41598-017-09037-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot analysis exhibiting levels of Ca²⁺ regulatory proteins as well as calcineurin A-NFAT signaling in myocardium from WT and Ctsk ^−/− mice treated with or without streptozotocin. (A) Representative gel blots of SERCA2a, phosphorylation of phospholamban (p-PLB), calcineurin A, phosphorylation of NFATc3, NFATc1 and GAPDH (loading control) using specific antibodies; (B) SERCA2a/GAPDH; (C) p-PLB/GAPDH; (D) calcineurin A/GAPDH; (E) p-NFATc3/GAPDH; (F) NFATc1/GAPDH. Mean ± SEM, n = 6 to 7 mice per group. ^*p < 0.05 vs. WT group, ^†p < 0.05 vs. WT-STZ group.