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. 2017 Aug 18;7:8703. doi: 10.1038/s41598-017-09037-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot analysis of Bax, Bcl-2, cleaved caspase-3, phosphorylation of AKT, AKT and GAPDH (loading control) in H9c2 cells treated with 25 mM high glucose in the present or absent of cathepsin K inhibitor CatK I-II or calcineurin inhibitor CsA. (A) Representative gel blots of Bax, Bcl-2, cleaved caspase-3, p-AKT, ATK and GAPDH (loading control) using specific antibodies; (B) cleaved caspase-3/GAPDH; (C) Bax/GAPDH; (D) Bcl-2/GAPDH; (E) Bax/Bcl-2 ratio; (F) p-AKT/GAPDH; (G) AKT/GAPDH; (H) p-AKT/AKT ratio. ^*p < 0.05 vs. LG group, ^†p < 0.05 vs. HG group.