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. 2017 Aug 18;7:8834. doi: 10.1038/s41598-017-07329-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of fasudil on retinal hypoxia. (A) Pimonidazole and lectin staining on flat-mounted retina from vehicle and fasudil treated rats. In hypoxic area stained with pimonidazole in the vehicle treated rats, lectin-labeled vessels appear constricted and irregular (a,b). Reduced hypoxic area observed in fasudil treated rats correspond to improved capillaries morphology (c) Magnification ×6. (B) Quantification of hypoxic aea shows that fasudil induces a significant reduction of hypoxic retinal surface ratio.