Table 1.
Phage display conditions used to retrieve FMDV binding VHHs.
| Llama | Coating |
Captured FMDV |
Competing FMDV |
Depleting FMDV |
VHHs selected | No. VHH sequenced | No. Unique VHHs | No. CDR3 groups | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Conc. (mg/l) | Protein | Conc. (mg/l) | FMDV antigen | Conc. (mg/l) | FMDV Antigen | Conc. (mg/l) | FMDV antigen | |||||
| 6058 | 1 | Streptavidin | 0.1 | Biotinylated Asia 1 Shamir antigen | – | None | – | None | M98 | 1 | 1 | 1 |
| 3050 | 0.1 | mAb13A6 | 0.5 | SAT2 SAU/2/00 146S | 10 | SAT2 SAU/2/00 12SA | – | None | M311–M317 | 7 | 5 | 4 |
| 3050 | 0.1 | M311F | 0.5 | SAT2 SAU/2/00 146S | – | None | 5 | SAT2 SAU/2/00 12SA | M371–M381 | 11 | 7 | 3 |
| 3049 | 0.1 | M98F | 0.5 | Asia 1 Shamir 146S | 10 | Asia 1 Shamir 12SA | – | None | M301–M308 | 8 | 8 | 7 |
| 3049 | 0.1 | M98F | 0.5 | Asia 1 Shamir 146S | – | None | 5 | Asia 1 Shamir 12SA | M331–M337 | 7 | 7 | 6 |
| 6058 | ||||||||||||
FMDV, foot-and-mouth disease virus.