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LOD of different DAS ELISAs.
| FMDV strain | DAS ELISA | LOD (μg/l) |
|
|---|---|---|---|
| Antigen | 12SH | ||
| SAT2 SAU/2/00 | M311F | 5.2 | 12 |
| SAT2 SAU/2/00 | M379F | 2.3 | 19 |
| Asia 1 Shamir | M98F | 3.8 | 19 |
| Asia 1 Shamir | M332F | 4.6 | >2,000a |
| Asia 1 Shamir | M3ggsVI4Q6E | 17 | 83 |
a>2,000 indicates LOD not reached at the highest VHH concentration used (2 mg/l).
DAS, double antibody sandwich; ELISAs, enzyme-linked immunosorbent assays; LOD, limit of detection.
