Table 2. Cross-talk matrix.
Detection windows, nm
|
||||
---|---|---|---|---|
530 | 560 | 580 | 610 | |
G-dye 1* | 1 | 0.75 | 0.39 | 0.13 |
A-dye 2 | 0.08 | 1 | 0.44 | 0.19 |
T-dye 3 | 0.02 | 0.19 | 1 | 0.38 |
C-dye 4 | 0.01 | 0.01 | 0.18 | 1 |
This table was obtained from a BigDye v3.1 Matrix Standard by using the Applied Biosystems datautility tool for the ABI PRISM model 377 DNA sequencer according to the manufacturer's protocol.
The chemical structures for BigDye v3.1 dyes have not been published to date. BigDye v1.1 structures are described in ref. 30.