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. 2017 Aug 18;7:8850. doi: 10.1038/s41598-017-09159-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Infection and wounding represses select HSR target genes at the site of injury. Graphical schematic of infection protocol (A). Levels of bacteria (B) or Gfp1 + S. marcescens (Db11) (C) relative to β-actin in abdominal wall (predominantly fat body) in uninjected bees (0) or those injected with either lysogeny broth (LB) or S. marcescens (Db11) four hours after injection. Transcript levels of Hymenoptaecin (D), Defensin 1 (E), and Abaecin (F) or Hsc70-4 (G), Hsp70Ab (H) and Hsp90 (I) relative to β-actin in abdominal wall (predominantly fat body) from these same bees. Bar and error bars represent the Mean ± SEM for expression values of the genes of interest calculated using the ΔΔC_T method. Statistical significance was assessed using one way ANOVA with Tukey’s multiple comparison test and is noted as *p < 0.05, and **p < 0.01.