Figure 2.

Overexpression of Rv2212 influences transition of M. tuberculosis to dormant “non-culturable” state. Recombinant Mtb strains containing pMindRv2212 vector (closed circles) or empty pMind vector (open circles) were grown in “glycerol” (A) or “glucose” (B) modified Sauton's media with agitation (200 rpm) at 37°C. For details see Materials and Methods. Minimum threshold for CFU determination was 10 cells per ml. SEM for the CFU determination was <20%. Experiments shown in (A) and in (B) were repeated two times and four times, respectively. Typical results are shown.