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. 2017 Aug 18;7:8780. doi: 10.1038/s41598-017-09310-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fundus pulsation analysis and repeatability measurements. (a) An exemplary fundus pulsation map overlaid on the corresponding reflectivity en-face projection at physiological IOP. Fundus pulsations were determined as velocity between retinal and chorioscleral complex. The velocities were averaged for each B-scan and subsequently analyzed by a STFT within a sliding window depicted in green. (b) The spectrogram showing the 15 spectral bands determined by the STFT and average spectral amplitude for the fundus pulsations shown in (a). The average spectral amplitudes were calculated for the fundamental oscillation as well as for the first harmonic. (c) The repeatability was investigated in a short time range of approximately 10 minutes (3 measurements each) as well as on a longer time span of approximately 40 minutes (total of 5 measurements each) for three rats. The normalized mean spectral amplitudes of the fundamental oscillation as well as of the first harmonic are shown, where error bars indicate the standard deviation.