Figure 5.

TAGLN2 is required for the activation of PI3 kinase/AKT and Ras-ERK signaling pathways. (a) Peritoneal macrophages from wild-type and TAGLN2 ^−/− mice were stimulated with LPS (1 μg/mL) for the indicated time points. The phosphorylated and total forms of AKT, ERK, and JNK were detected by western blot. (b) Peritoneal macrophages were pre-activated with LPS (1 μg/mL) for 24 h. Cells were serum-starved for 3 h and stimulated with fMLP (1 μM) for the times indicated. Cell lysates were subjected to western blot with anti-phospho and total PI3K, AKT, ERK, and JNK. The data are representative of three independent experiments. Full-length blots/gels are presented in Supplementary Fig. S9.