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. 2017 Aug 18;7:8808. doi: 10.1038/s41598-017-08818-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Influence of increasing amounts of ecoTatA on thylakoid transport mediated by peaTatA. In thylakoido complementation of 16/23 transport by peaTatA was analysed in the presence of increasing amounts of ecoTatA. Both TatA proteins were obtained from in vitro translation in the RTS system in the presence of [³⁵S]-methionine, which allows their detection by autoradiography (stars: peaTatA, diamond: ecoTatA). The composition of each supplementation, i.e. the proportion of pea TatA, eco TatA, and empty RTS in the total volume of 25 μl, is given above the lanes. Ti-1 indicates the position of the protease-protected fragment indicative of translocation intermediate Ti-1 of the 16/23 chimera which represents the membrane-bound precursor protein prior to membrane translocation of the 23 kDa passenger polypeptide^{24, 48, 55}. For further details see the legend to Fig. 2.