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. 2017 Aug 18;7:8855. doi: 10.1038/s41598-017-09592-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Engineering CYP76AH24 specificity. (A) Model of CYP76AH24 showing the residues selected for mutagenesis (F112, V296 and F477). Graphic produced by DeepView (Swiss-PdbViewer). (B) Product profile of selected mutants. Products include: ferruginol (green), 11-hydroxy-ferruginol (red) and 11β-hydroxy-manoyl oxide (blue). Two variants, F112L and V296L, showed substrate selectivity and improved catalytic activity. Product titers for each of the three substrates were normalized based on the corresponding wild-type CYP76AH24 yield listed in Table S1 and considered as 100%.