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. 2017 Aug 18;7:8855. doi: 10.1038/s41598-017-09592-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Engineering the specificity of CYP76AH24. (A) Evaluation of CYP76AH24 variants in miltiradiene producing yeast cells (V296L (teal) and F112L (pink) versus wt (black)). (B) Evaluation of the same CYP76AH24 variants in manoyl oxide producing yeast cells. (C) Steady-state kinetic analysis of the oxidation of abietatriene by CYP76AH24 variants V296L (open squares) and F112L (open circles) versus wt (closed circles).