Table 3.
Kinetic parameters of the engineered CYP76AH24 variants.
| Oxidized carbon | Substrate | Enzyme | k cat (min−1) | K M (μM) | k cat/K M (× 103 min−1 M−1) | Source |
|---|---|---|---|---|---|---|
| C-12 | abietatriene | CYP76AH24 | 5.51 ± 0.71 | 20.62 ± 6.12 | 267.2 | ref. 19 |
| CYP76AH24(V296L) | 6.24 ± 0.93 | 20.03 ± 6.97 | 311.5 | this study | ||
| CYP76AH24(F112L) | 9.69 ± 1.31 | 15.67 ± 5.53 | 618.4 | this study | ||
| C-11 | ferruginol | CYP76AH24 | 6.11 ± 0.45 | 45.84 ± 9.37 | 133.3 | ref. 19 |
| C-2α | ferruginol | CYP71BE52 | 0.04 ± 0.002 | 3.96 ± 0.72 | 10.1 | ref. 19 |
| C-20 | ferruginol | CYP76AK6 | 1.43 ± 0.21 | 20.81 ± 7.76 | 68.7 | ref. 19 |