Fig. 6.

Total DNAPKcs levels are unchanged by RT treatment, but treatment with MDV3100 decreased phosphorylated DNAPKcs levels after ionizing radiation. Total DNAPKcs and phosphoDNAPKcs levels were measured ±RT and ±MDV3100 treatment at 1, 2, 5, 10, and 30 min (a). Quantification of phosphoDNAPKcs changes ±MDV3100 radiation treatment are depicted (b). Mechanistically, AR functions to allow for active phosphorylation of DNAPKcs after leading to more efficient resolution of dsDNA breaks induced by ionizing radiation. This activation is impaired by antiandrogen therapy (c). Experiments were repeated in triplicate and error bars represent SD