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. 2017 Aug 18;7:8729. doi: 10.1038/s41598-017-08663-x

Figure 7.

Figure 7

Rap1 protects the 5′ end when its binding site contains 2 nt but not 4 nt of ssDNA. (a) Telomere part of D13S13 contains 13 bp dsDNA and a 13 nt 3′ overhang. The Rap1 MBS (bold text) is located across the ds-ss junction and contains 2 nt of ssDNA. (b) D11S15 telomere part has 11 bp dsDNA and a 15 nt 3′ overhang. The Rap1 MBS locates across the ds-ss junction and contains 4 nt of ssDNA. The black bar indicates the guide sequence, and “*” the radioactive label (as described in Fig. 2 legend). (c, d) DEPA gels showing the results from D13S13 (c) and D11S15 (d). Both (c) and (d) show the loading control (LC) and uncleaved substrate (S) from the Rap1 containing and BSA control reactions at 0; 20; 40; 60; 120 s. Uncropped gels can be found in Supplementary Fig. S4. (e,f) Quantification of gels shown in (c) and (d) respectively.