Table 1. Measured values of ΔG, Keq, and KD.
| WASP | ΔG, kcal·mol-1 | Keq | KD, μM |
|---|---|---|---|
| BGBD-C | -5.5 | 11,000 | 98 ± 13 |
| GBD—VCA | -4.2 | 1,200 | 114 ± 8 |
| M307A (GBD—VCA) | -3.4 | 310 | 71 ± 5 |
| GBDPVCA | -3.3 | 260 | n.d. |
| M307K (GBD—VCA) | -2.4 | 58 | 13 ± 1 |
| 1290A (GBD—VCA) | -1.5 | 13 | 4.0 ± 0.1 |
| 1294A (GBD—VCA) | -0.4† | 2 | 0.50 ± 0.05 |
| 1290Q (GBD—VCA) | n.d. | n.d. | 0.20 ± 0.04 |
| M307A (BGBD—VCA) | -3.9 | 730 | n.d. |
| M307K (BGBD—VCA) | -2.8 | 110 | n.d. |
| 1290A (BGBD—VCA) | -2.5 | 68 | n.d. |
| 1294A (BGBD—VCA) | -0.9† | 5 | n.d. |
n.d., not determined.
†
This measurement is only approximate and may not represent the unfolding of tertiary structure.