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. 2017 Aug 18;7:8777. doi: 10.1038/s41598-017-09138-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TLC analysis of different metabolites of Alternaria displaying different spots on TLC plates. (A) showing the spots of alternariol monomethyl ether (AME), (B) showing the spots of alternariol (AOH), and (C) showing the spots of tenuazonic acid (TeA), on the basis of their R_f values. The standards of these toxins were also run on similar plates for comparisons of the R_f values. The spots were visualized by spraying ferric chloride (FeCl₃) solution or under UV-light.