Table 3.
Comparisons of Rf value of the phytotoxins isolated from Alternaria species with standards of tenuazonic acid, alternariol, alternariol monomethyl ether by TLC
| Rf values | Chloroform: methanol (80:20) | Benzene: acetone: acetic acid (60:35:5) | Chloroform: methanol (95:5) | Ethyl acetate: benzene (95:5) |
|---|---|---|---|---|
| Standard TeA | 0.63 ± 0.02 | 0.56 ± 0.03 | 0.42 ± 0.04 | 0.23 ± 0.05 |
| Isolated toxins | 0.62 ± 0.03 | 0.55 ± 0.01 | 0.41 ± 0.07 | 0.22 ± 0.06 |
| Standard AOH | 0.66 ± 0.09 | 0.59 ± 0.04 | 0.49 ± 0.03 | 0.31 ± 0.01 |
| Isolated toxins | 0.67 ± 0.04 | 0.60 ± 0.02 | 0.48 ± 0.04 | 0.32 ± 0.02 |
| Standard AME | 0.82 ± 0.01 | 0.78 ± 0.05 | 0.61 ± 0.09 | 0.39 ± 0.04 |
| Isolated toxins | 0.81 ± 0.06 | 0.77 ± 0.04 | 0.60 ± 0.02 | 0.38 ± 0.05 |
Note: Rf value is the ratio of the distance travelled by the substance and the distance travelled by the solvent (The spots were developed by spraying the plates with 0.2% FeCl3 in ethanol and some are detected in UV-light at 365 nm).