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. 2017 Aug 18;7:8710. doi: 10.1038/s41598-017-09504-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MSI1 increased the aspect ratio and decreased viscoelasticity of GBM cells. (A)(B) MSI1-depleted 05MG cells and MSI1-overexpressed U251 cells were immune-stained for F-actin (Red) and DAPI (Blue) and observed by fluorescent microscopy. The ratio of mean aspect (defined as the ratio of the length of major and minor axes, AR) were shown in the right (n = 100). (C)(D) Fluorescent color maps of F-actin networks distribution in MSI1-depleted 05MG cells and MSI1-overexpressed U251 cells. The formation and the angular distribution of F-actin orientation in MSI1-depleted 05MG and MSI1-overexpressed U251 cells were analyzed by assessing and quantifying the immunofluorescence intensities using MatLab software. The mean of Kurtosis from 45 cells was shown in the right. (E) (F) The intracellular elastic modulus (G’) and viscous modulus (G”, at 10 Hz) of MSI1-depleted 05MG and MSI1-overexpressed U251 cells were shown as mean ± SEM (n = 100 beads/ 35cells). Each bar chart is presented as mean ± SEM. The experiments were repeated at least three times. The statistical significance was assessed by one-way ANOVA, *P < 0.05 (relative to the control group).