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. 2017 Aug 18;7:8710. doi: 10.1038/s41598-017-09504-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene ontology (GO) and pathway analyses for MSI1 targets distribution in actin rearrangement. Ribonucleoprotein immunoprecipitation sequencing (RIP-seq) of MSI1-associated RNAs were performed in 05MG cells. (A) Gene ontology (GO term) enrichment analysis was conducted by DAVID software per the biological processed. (B) MSI1-immunoprecipitated mRNAs are enriched for protein-protein interaction network, of which the top-ranked genes from the GO terms were listed. (C) Non-significant genes were cut off, narrowing down the original 22667 genes into 2286 target genes. TNS3 was found in the highly expressed pool of genes. (D) The top 10 significantly changed genes were analyzed by REVIGO (http://revigo.irb.hr/) and listed in the chart. The numbers on each graph showed the corresponding GO term of cell migration and its read counts by FPKM (fragments per kilobase of exon per million fragments mapped). (E) Endogenous MSI1 was precipitated from 05MG cells treated without or with RNaseA (10 mg/ml). The precipitated complexes were subjected to RT-PCR to assess TNS3 mRNA levels and by Western blotting to confirm the MSI1 precipitation, of which the uncropped blots were demonstrated in Supplementary Figure 5.