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. 2017 Aug 18;7:8712. doi: 10.1038/s41598-017-09143-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Target assay of effect of miRNAs on the predicted target gene expression in rice protoplasts. (a) Fluorescence micrographs of rice protoplasts transfected with YFP, YFP/miR160f-5p, ARF16-YFP and ARF16-YFP/miR160f-5p from left to right. The photographs were taken at 60 × magnification. (b) Fluorescence micrographs of rice protoplasts transfected with YFP, YFP/miR167a-5p, NB-ARC-YFP and NB-ARC-YFP/miR167a-5p from left to right. The photographs were taken at 60 × magnification. (c) Western blot analysis of ARF16 expression or black plasmid expression in rice protoplasts transfected with YFP, YFP/miR160f-5p, ARF16-YFP and ARF16-YFP/miR160f-5p from left to right using anti-HA and anti-GAPDH antibodies. ARF16 is indicated with arrow. (d) Western blot analysis of NB-ARC expression or black plasmid expression in rice protoplasts transfected with YFP, YFP/miR167a-5p, NB-ARC-YFP and NB-ARC-YFP/miR167a-5p from left to right using anti-HA and anti-GAPDH antibodies. NB-ARC is indicated with arrow. The original blots of c and d are shown in Fig. S5.