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. 2017 Jan 25;10(5):1145–1159. doi: 10.1038/mi.2016.139

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Copyright © 2017 The Author(s)

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Maintenance mechanisms of corneal macrophages at steady state or after corneal wounding. (a) Costaining of cornea from 8-week-old mice with CD64-PE and Ki-67-FITC (the upper three images) (bars=50 μm). Flow cytometric analysis of the percentage of Ki-67-positive cells in CCR2⁻ and CCR2⁺ corneal macrophages (lower three images). Control isotype antibodies are shown on the left. (b) Staining of cornea from recipient female mice with anti-mouse CD64-PE (bars=100 μm). (c) Percentage of YFP⁺ cells in corneal macrophages from wild and recipient mice at steady state, and (d) after corneal epithelial wounding. All samples in this figure were obtained at the same time. FITC, fluorescein isothiocyanate; IgG, immunoglobulin G; PE, phycoerythrin; SSC, side scatter; YFP, yellow fluorescent protein.