Fig. 1.

Chemiluminescence signal after assay in 3C for paired injections, as indicated, of a GSNO dilution series (without CO) (series A), or the same injections of GSNO as for series A after preinjection of NO-depleted Hb into the reflux chamber (series B). The mixture of Hb and GSNO in the reflux chamber simulates the noted SNO/Hbs; the signal for GSNO is lost, presumably to capture of NO by heme Fe. (series C) The signal for GSNO as in series A returns after adding carbonyl-purged CO to the inert gas stream, resolving the signal attenuation shown in series B.