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. 2017 Aug 18;7:8732. doi: 10.1038/s41598-017-08230-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AGB1, GPA1, AGG1 and AGG2 interact with MPK6 and MPK3. (A–J) Tobacco leaves co-infiltrated with agrobacterium containing 35S-driven split luciferase (LUC) constructs as indicated were photographed with a charge-coupled device camera. Each image is representative of three images in three independent experiments. (A–E) MPK6-nLUC interacts with cLUC-AGB1 (A), cLUC-WtGPA1 (B), cLUC-AGG1(C), cLUC-AGG2 (D), cLUC-GPA1^Q222L (E), respectively. (F–J) MPK3-nLUC interacts with cLUC-AGB1 (F), cLUC-WtGPA1 (G), cLUC-AGG1 (H), cLUC-AGG2 (I), cLUC-GPA1^Q222L (J), respectively. The pseudocolor bar shows the relative range of luminescence intensity in images. Pull-down assay shows that AGB1, WtGPA1, GPA1^Q222L, AGG1 and AGG2 interact with MPK6 (K), respectively. AGB1 and GPA1 interact with MPK6 (L), but not MPK4 (M) by Co-IP assay. Full blots are shown in Supplemental Data.