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. 2017 Aug 18;7:8732. doi: 10.1038/s41598-017-08230-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bimolecular fluorescence complementation analysis showing the interaction between AGB1, MPK6 and MKK5. Images of tobacco leaf sections expressing CFP^C-AGB1 and CFP^N-MPK6 (A), CFP^N-AGB1 and CFP^C-MKK5 (D) exhibit fluorescence, but not CFP^C-AGB1 and CFP^N (B), CFP^C and CFP^N-MPK6(C), CFP^N-AGB1 and CFP^C (E), or CFP^N and CFP^C-MKK5 (F). Each image is a representative of three images in three independent experiments.