Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 18;7:8732. doi: 10.1038/s41598-017-08230-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

AGB1 and SSP physically interact with the N- and C-termial of YODA by luciferase complementation assay and pull-down assay. (A) Protein structure of YODA. N-Tail, kinase, C-Tail and Δ334 (334–883aa) are indicated in the diagram. (B–F) Tobacco leaves co-infiltrated with agrobacterium containing 35S-driven construct pairs as indicated were photographed with a charge-coupled device camera. (B–F) cLUC-AGB1 interacts with nLUC-YDA-N-Tail (C) and nLUC-YDA-C-Tail (F), but not nLUC-YDA (B), nLUC- YDA-Kinase (E) or nLUC-YDA-Δ334 (D), respectively. (G–K) cLUC-SSP interacts with nLUC-YDA-N-Tail (H) and nLUC-YDA-C-Tail (K), but not nLUC-YDA (G), nLUC-YDA-Kinase (J) or nLUC-YDA-Δ334 (I), respectively. The pseudocolor bar shows the relative range of luminescence intensity in images. Pull-down assay shows that AGB1 (L) and SSP (M) interact with the N-Tail and C-Tail of YODA, but not the kinase domain. GST does not interact with the N-Tail, kinase domain or C-Tail of YODA (N). Full blots are shown in Supplemental Data.