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. 2017 Aug 18;7:8824. doi: 10.1038/s41598-017-08869-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of hiPS-CMs and hiPS-CM cell sheet. (a) Expression of cardiac troponin T (cTNT) after differentiation and purification of hiPS-CMs was determined by flow cytometry anaysis. (b) After differentiation and purification, sarcomere structure was visualized by sarcomeric alpha actinin staining in hiPS-CM. (c) Immunostaining of the hiPS-CM cell sheet with cTNT antibody (green). The cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 10 μm in (b) and 100 μm in (c).