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. 2017 Aug 18;7:8846. doi: 10.1038/s41598-017-08691-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of light on the suprachiasmatic nuclei. (a) Representative photomicrographs showing Fos-immunoreactivity in the suprachiasmatic nuclei (SCN) of degus (left panel) and rats (right panel) under each experimental condition: control animals, which received no light pulse (darkness), and after 1-hour light pulse at CT16 with RGB or RGV lights. The SCN area is delimited by dotted lines. (b) Quantification of Fos-immunoreactive neurons (counted on the left part of the SCN) in the control group (no light pulses, n = 9 degus; 4 rats), the RGB group (pulsed for 1 h with RGB light at CT16, n = 7 degus; 4 rats) and the RGV group (pulsed for 1 h with RGV light at CT16, n = 7 degus; 4 rats). Data are expressed as the mean ± SEM. *Indicates significant differences compared to control degus (Kruskal-Wallis, p = 0.030; Mann-Whitney U test, p = 0.034).