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. 2017 Aug 18;7:8814. doi: 10.1038/s41598-017-06851-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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p21, MGMT and TP53 expression in U87, U373, T98 and TR-U373 cells with TMZ or GADD45A siRNA treatment. Analysis of the expression of p21 in U87, U373, TR-U373 and T98 cells treated with TMZ (25 μg/ml) or GADD45A siRNA. (a) Analysis of p21 gene expression in GBM cell lines by qPRC. n = 3, *p < 0.05 (b) Analysis of p21 protein expression in GBM cell lines by western blotting. Original blot was shown in Supplementary Fig. 8. (c) The mgmt gene expression in GBM cell lines analyzed by RT-PCR. Full-length gels were shown in Supplementary Fig. 4. (d) Analysis of TP53 protein expression in GBM cell lines by western blotting. Original blot was shown in Supplementary Fig. 9. n = 3, *p < 0.05.