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. Author manuscript; available in PMC: 2017 Aug 19.
Published in final edited form as: Cell Rep. 2017 Apr 4;19(1):86–100. doi: 10.1016/j.celrep.2017.03.028

Figure 7. Distal axon-derived TrkA signals locally, within dendrites, to maintain PSDs in sympathetic neurons.

Figure 7

A. Biotinylated-PLGA-1NMPP1 microspheres were applied to the somatodendritic compartment of DIV 14 compartmentalized TrkAF592A sympathetic neurons cultured on coverslips pre-coated with streptavidin while NGF was applied to distal axons to achieve localized TrkA kinase inhibition pockets adjacent to dendrites. B. Bright field image of SCG neurons. Arrows denote PLGA-1NMPP1 microspheres. C. P-TrkA and MAGUK puncta in dendrites of neurons treated with PLGA (left) or PLGA-1NMPP1 (right) microspheres. White circles are drawn over microspheres. D. P-TrkA puncta in soma of neurons treated with PLGA (left) or PLGA-1NMPP1 (right) microspheres, quantified in G. E and H. Quantification of the distance between PLGA or PLGA-1NMPP1 microspheres and the nearest P-TrkA (D) or MAGUK (G) puncta; Welch’s t-test*p<0.05. F and I. Quantification of the distance between each PLGA microsphere and dendrite mask per experiment. Data are presented as mean ± SEM. See also Figure S6.