Fig. 5.

Cuprizone treatment leads to inhibitory synaptic changes in the lateral geniculate nucleus. a Bar graph depicting the number of puncta immuno-positive for the vesicular GABA transporter vGAT (pre-synaptic, inhibitory) in the dLGN at 5 weeks of cuprizone treatment. Control levels were set to 1.0 (dotted line). Two fields per slice and two slices per animal (n = 3: control and cuprizone) were analyzed. b Representative images showing the distribution of vGAT-positive synaptic densities at 5 weeks of cuprizone treatment (right panel) or under control conditions (left panel). Scale bar: 20 μm. The bar graph depicts mean ± SEMs. *p < 0.05, **p < 0.01, ***p < 0.001, not significant (ns) p ≥ 0.05 (one-sample t test; compared to set control value = 1)