Fig. 3.

CIN85 (A) and Cbl (B) are pulled down by domains of ICP0 containing SH3 domain binding sites. GST-ICP0 chimeric proteins bound to agarose beads as described in Materials and Methods were reacted with equal amounts of uninfected HEK293 cell lysates. The proteins bound to the beads were solubilized, separated in denaturing gels, transferred to a nitrocellulose membrane, and reacted with antibodies against CIN85 (A Upper) or GST (A Lower), Cbl (B Upper) or stained with Ponceau S (B Lower). The dots identify the bands that contain full-size GST-ICP0 chimeric proteins.