Table 4.

Proteins differentially expressed under stress conditions in strains CcI6 and CcI3

	SPOT #	Acc. No	Locus Tag	Protein Name	MW (Da)	PI	NaCl	Sucrose
[C] Energy production and Conversion
CcI6	10	563,313,506	CCI6_RS13315	electron transfer flavoprotein alpha subunit apoprotein	32,842.70	5.01	↓	↓
	15	563,312,797	CCI6_RS16290	NAD-dependent aldehyde dehydrogenase	54,456.60	5.40	N/C	↓
	1	563,313,455	CCI6_RS13080	ATP synthase F1 subcomplex beta subunit	56,735.20	4.56	↓	N/C
	8	563,314,955	CCI6_RS05745	malate dehydrogenase (NAD)	34,399.10	4.96	↑	N/C
[E] Amino acid transport and metabolism
CcI6	2	563,312,117	CCI6_RS19490	L-glutamine synthetase	53,786.20	4.97	↑	N/C
	10	563,315,075	CCI6_RS05150	cysteine synthase (CysK)	32,443.60	4.95	↑	↓
[G] Carbohydrate transport and metabolism
CcI6	5	563,312,326	CCI6_RS18410	glyceraldehyde-3-phosphate dehydrogenase (NAD+)	35,515.80	5.76	↑	N/C
	7	563,314,408	CCI6_RS08915	fructose-bisphosphate aldolase	36,894.20	5.35	↑	↑
CcI3	4	WP_011436076.1	FRANCCI3_RS08225	glyceraldehyde-3-phosphate dehydrogenase (NAD+)	35,515.80	5.76	↑	N/C
	5	WP_011438718.1	FRANCCI3_RS22085	fructose-bisphosphate aldolase	36,894.20	5.35	↑	N/C
	11	WP_011437192.1	FRANCCI3_RS14110	aldolase	38,526.7	5.3	N/C	↑
[H] Coenzyme metabolism
CcI6	3	563,314,085	CCI6_RS10325	methionine adenosyltransferase	42,893.60	5.05	↑	N/C
	9	563,313,788	CCI6_RS11580	pyridoxal phosphate synthase yaaD subunit	32,577.80	5.34	↑	↑
CcI3	9	WP_011437570.1	FRANCCI3_RS16030	6,7-dimethyl-8-ribityllumazine synthase	16,137.8	5.5	↑	N/C
[I] Lipid transport and metabolism
CcI3	3	WP_011438063		acetyl-CoA acetyltransferase	39,714.5	5.3	↑	N/C
[J] Translation
CcI3	8	WP_011437955.1	FRANCCI3_RS18065	ribosome-recycling factor	20,830.7	5.4	↑	↑
[K] Transcription
CcI6	3	563,314,632	CCI6_RS07960	DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)	44,399.30	5.10	↑	N/C
	12	563,312,999	CCI6_RS15595	RNA polymerase, sigma 24 factor	29,218.90	5.49	↓	N/C
	13	563,314,238	CCI6_RS09105	Transcriptional regulator Crp/Fnr	51,393.10	4.97	↓	N/C
CcI3	14	WP_011437589.1	FRANCCI3_RS16125	XRE family transcriptional regulator	17,993.4	5.7	↑	N/C
[M] Cell wall/membrane/envelop biogenesis
CcI6	6	563,313,716	CCI6_RS12035	UDP-glucose pyrophosphorylase	34,856.10	5.04	↑	↑
	11	563,315,562	CCI6_RS03270	Nucleoside-diphosphate-sugar pyrophosphorylase family protein	31,492.90	4.92	↑	N/C
[O] Post-translational modification, protein turnover, chaperone functions
CcI6	2	563,314,374	CCI6_RS08745	chaperonin GroEL	56,735.20	4.72	↑	N/C
	14	563,311,297	CCI6_RS22940	ATP-dependent Clp protease proteolytic subunit ClpP	23,039.30	4.79	↑	↑
[R] General Functional Prediction only
CcI6	16	563,312,796	CCI6_RS16285	Zn-dependent alcohol dehydrogenase	35,062.50	5.65	N/C	↓
CcI3	7	WP_011434678.1	FRANCCI3_RS01050	FMN reductase	21,222.1	5.3	↑	↑
Not assigned to COG categories
CcI6	4	563,313,603	CCI6_RS12255	Nitroreductase	37,351.40	4.90	↑	↑
	12	563,313,563	CCI6_RS12810	proteasome endopeptidase complex	28,833.80	5.11	↑	N/C

The identified proteins were classified by COG functional categories. More than one protein per spot has been identified for some spots. Upregulated proteins are shown by the upward pointing arrow (↑) whereas downregulated proteins are shown by the downward pointing arrow (↓). No change (N/C) indicates that a spot was not picked for that particular condition because it showed similar intensity as the control