Table 2.
Gene primers tested in comparative RT-PCR experiments
| Gene | Sense | Antisense |
|---|---|---|
| CD86 | 5′-TGCTCATCTAAGCAAGGATACCCG-3′ | 5′-CGACTCGTCAACACCACTGTCCTG-3′ |
| CD206 | 5′-GGATTGTGGAGCAGATGGAAG-3′ | 5′-CTTGAATGGAAATGCACAGAC-3′ |
| p22PHOX | 5′-GACGCTTCACGCAGTGGTACT-3′ | 5′-CACGACCTCATCTGTCACTGG-3′ |
| p47PHOX | 5′-CAGAATGTTGCCTGGTTG-3′ | 5′-GTCCCCTCCCTTAGATGA-3′ |
| gp91PHOX | 5′-CTTCACACGGCCATTCACAC-3′ | 5′-GTCATAGGAGGGTTTCCGGC-3′ |
| TNFα | 5′-GGCAGCCTTGTCCCTTGAAGAG-3′ | 5′-GTAGCCCACGTCGTAGCAAACC-3′ |
| NFκB | 5′-GTGCAGAAAGAAGACATTGAGGTG-3′ | 5′-AGGCTAGGGTCAGCGTATGG-3′ |
| iNOS | 5′-CAGCCCTCAGAGTACAACGAT-3′ | 5′-CAGCAGGCACACGCAATGAT-3′ |
| Arg-1 | 5′-CAGTATTCACCCCGGCTACG-3′ | 5′-GCCTGGTTCTGTTCGGTTTG-3′ |
| CD68 | 5′-TGTACCTGACCCAGGGTGGAA-3′ | 5′-GAATCCAAAGGTAAGCTGTCCGTAA-3′ |
| IL10 | 5′-CAAGGAGCATTTGAATTCCC-3′ | 5′-GGCCTTGTAGACACCTTGGTC-3′ |
| GAPDH | 5′-GCTGGTCATCAACGGGAAA-3′ | 5′-ACGCCAGTAGACTCCACGACA-3′ |
Table provides sense and antisense sequences for each gene tested in this study. Genes investigated that showed no significant difference by age in either naïve or injured animals were omitted from results