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. 2017 Aug 18;13:259. doi: 10.1186/s12917-017-1173-6

Table 1.

Results of the analysis of boot swabs and liquid manure samples (n/ %) from 77 herds by faecal culture in three labs (I to III) and by two different qPCR methods (qPCR A and qPCR B) for Mycobacterium avium ssp. paratuberculosis (MAP)

Methode of MAP detection Laba Boot swab Liquid manure
posb negc n.a.d pos neg n.a.
Faecal culture I 40/ 51.9 37 /48.0 0 /0.0 39 /50.6 36 /46.8 2 /2.6
Faecal culture II 41/ 53.2 36 /46.8 0 /0.0 38 /49.4 37 /48.0 2 /2.6
Faecal culture III 41 /53.2 35 /45.5 1 /1.3 21 /27.3 54 /70.1 2 /2.6
qPCR A II 35 /45.5 39 /50.6 3 /3.9 33 /42.9 35 /45.5 9 /11.7
qPCR B III 37 /48.0 36 /46.8 4 /5.2 38 /49.4 32 /41.6 7 /9.1

aLaboratory

bMAP-positive

cMAP-negative

dNon-assessable faecal culture due to contamination or non-assessable qPCR results