Fig. 7.

HAdV-B3 and MAdV-1 hexon Abs do not reveal neutralizing activity. a Five-fold serial dilutions of preimmune serum or HAdV-B3 hexon Ab were pre-incubated with HAdV-B3-CMVLuc for 30 min before adding to human A549 cells. b Five-fold serial dilutions of preimmune serum or MAdV-1 hexon Ab were pre-incubated with MAdV-1-E1A-FS2A-GL for 30 min before adding to mouse CMT93 cells. For both viruses, luciferase activity was measured as RLU in lysed cells 24 h post infection. c Comparison of the neutralizing activities of the HAdV-3 hexon Ab with a neutralizing rabbit anti HAdV-B3 serum. Dilutions and experimental set-up were as described above