Table 1.
Details of primers used in RT- qPCR
| Gene | Oligo sequence | Product size (bp) | Annealing temperature (°C) | Genbank accession no. |
|---|---|---|---|---|
| EF-1α | F: GATCCAGAAGGAGGTCACCA R: TTACGTTCGACCTTCCATCC |
150 | 58 | NM_001124339.1 |
| β-actin | F: CAGCCCTCCTTCCTCGGTAT R: AGCACCGTGTTGGCGTACA |
110 | 54 | NM_001124235.1 |
| TLR-2 | F: GATCCAGAGCAACACTCTCAACAT R: CTCCAGACCATGAAGTTGACAAAC |
282 | 58 | LK933545.1 |
| C3 | F: GAGATGGCCTCCAAGAAGATAGAA R: ACCGCATGTACGCATCATCA |
91 | 58 | L24433.1 |
| IL1β | F: GACATGGTGCGTTTCCTTTT R: ACCGGTTTGGTGTAGTCCTG |
122 | 54 | AJ278242.2 |
| IgT | F: AACATCACCTGGCACATCAA R: TTCAGGTTGCCCTTTGATTC |
80 | 54 | AY870266.1 |
| IgM | F: TGCGTGTTTGAGAACAAAGC R: GACGGCTCGATGATCGTAAT |
107 | 54 | AH014877.2 |
| CD4-1 | F: GAGTACACCTGCGCTGTGGAAT R: GGTTGACCTCCTGACCTACAAAGG |
121 | 58 | NM_001124539.1 |
| CD8a | F: ACGACTACACCAATGACCACAACC R: CAGTGATGATGAGGAGGAGGAAGA |
160 | 58 | AF178055.1 |